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Welcome to the Barile Lab

Enter here to discover the world of food components elucidated through mass spectrometry.

What we do

  • Bioactive Compound Isolation and Characterization, Protein/glycoprotein chemistry, purification and characterization, Glycomics, Methods Development, Collaboration with Industrial/Academic partners

Our thematic areas of research

  1. Lab-scale analytical discovery of complex glycans in foods and food streams
  2. Pilot-scale engineering to separate newly discovered glycans and produce unique food ingredients
  3. In vitro and in vivo studies to establish safety and elucidate the potential health benefits of the isolated complex glycans.

Accomplishments

  • Our research explores how novel ‘omics combined with advances in separation science makes possible discoveries of bioactive food components.
  • Using a combination of Proteomics, Glycomics, Lipidomics and Peptidomics in a cheese whey model system, our work captures previously unavailable bioactive milk components that can be rapidly adapted into novel functional foods while adding value to a waste stream.
  • With successful separation of milk components and verification of their similarity to human milk, these can serve as the basis for improved infant formulas and other therapeutic applications.
  • Based on this foundation, we recently assembled a comprehensive structural bioinformatic library for bovine milk oligosaccharides. This library includes several novel bioactive (fucosylated) oligosaccharides and will allow rapid identification of low-abundant oligosaccharides in all food streams.
  • Currently, the dairy industry must manage the cost of discarding whey permeate as a waste stream from whey protein concentrate production. Capturing the value from this waste stream as a high-value supplement and/or product for both targeted at-risk populations and for the general public could result in significant, direct economical revenue.
  • We are conducting the first human study to elucidate tolerability and effectiveness of bovine milk oligosaccharides in enriching intestinal microflora toward beneficial populations (ClinicalTrials.gov Protocol Record 264294 )
  • Our ultimate goal is to make significant contributions to the development of novel processes that reduce agricultural/industrial waste with the aim of recovering from those side-streams important bioactive components that can be used to improve health.

Our cutting edge technologies

  • Nano LC MicroChip Quadrupole ToF (Time of Flight)
  • MALDI ToF -Matrix-assisted laser desorption/ionization Time of Flight
  • HPAE-PAD - High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection
  • The combined use of these 3 analytical instruments provides a platform of technologies upon which we can investigate relatively untapped organic “waste” streams for valuable, healthful components, with concomitant mitigation of environmental pollution.

Current Projects

  • Pilot study on the tolerability of feeding different doses of bovine milk oligosaccharides for modifying gastrointestinal function in healthy humans
  • Revealing the Function of Bovine Milk Oligosaccharides in the Human Gut Using Metatranscriptomics
  • Mechanisms by which human milk peptides and N-glycans shape the infant intestinal microbiota
  • Separation and purification of oligosaccharides from human milk
  • Protecting the fragile intestine: integrating microbiota and mucosal health
  • Integrating Biology, Psychology, and Ecology to Mitigate Antibiotic Resistance in Food Animal Production Systems
  • The Breast Milk, Gut Microbiome and Immunity (BMMI) Project
  • Oligosaccharide profiles of cow’s milk for the optimization of health promoting attributes
  • Development of a bioprocessing method to recover heath enhancing components from agricultural waste streams
  • Enzymatic modification of isolated bovine milk oligosaccharides to mimic human milk oligosaccharides with increased antimicrobial activity
  • Activation of Probiotic Bifidobacteria Effects by Milk Glycans
  • Diversity of oligosaccharides in milk and milk replacer fed to calves and their effect on diversity of fecal Bifidobacteria
  • Advanced strategies for production and characterization of novel prebiotic oligosaccharides
  • Polysaccharides from non-Saccharomyces yeasts: a new opportunity to improve wine quality and human health
  • Quantitative analysis of bovine milk peptides, oligosaccharides and glycolipids in dairy streams

Feel free to contact us if you have any questions.

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